Establishment of PCR System for Rapid Detection of Four Kinds of Common Aquatic-Food Pathogens

Abstract

In this paper, using polymerase chain reaction (PCR) detection method, with sets of primers from the tlh, tdh, trh, hly, inv A, rfbe, stx 1, stx 2 of Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella and Escherichia Coli were designed to establish a novel method for rapid detection of common aquatic-food pathogens. The results showed that the new established PCR system was 100% specificity and using this method was capable of detecting a minimum of 8.28×10¹ CFU/mL for genomic DNA. This method was directly applied in the aquatic samples and the detection limit was be 4×10³ CFU/mL, 2.14×10³ CFU/mL, 8×10⁴ CFU/mL and 1.07×10³ CFU/mL respectively. It could be detected in 3hours. It reveals that a rapid PCR technique for the detection of common aquatic-food pathogens was established successfully.